Article: cAMP response element-binding protein-mediated gene expression increases the intrinsic excitability of CA1 pyramidal neurons.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) Lopez de Armentia M; Jancic D; Olivares R; Alarcon JM; Kandel ER; Barco A J. Neurosci., 2007
Group of mice (n = neurons, mice) | Em (mV) | Rin (MΩ) | τ (ms) | AP amplitude (mV) | AP50 (ms) | dV/dtmax (V/s) | Threshold (mV) | Medium IAHP (pA) | Slow IAHP (pA) |
---|---|---|---|---|---|---|---|---|---|
VP16–CREBhigh On (n = 22, 7) | −56 ± 1.1 | 210 ± 20.7 | 21 ± 1.6 | 112 ± 1.6 | 0.99 ± 0.05 | 251 ± 10.4 | −33 ± 1.2 | 120 ± 13.1 | 15 ± 2.5 |
Wild-type On (n = 16, 5) | −58 ± 2.1 | 181 ± 17.3 | 25 ± 2.7 | 119 ± 1.3 | 1.01 ± 0.03 | 309 ± 9.8 | −39 ± 0.7 | 251 ± 19.8 | 41 ± 6.7 |
p | 0.40 | 0.29 | 0.13 | 0.004 | 0.71 | <0.001 | <0.001 | <0.001 | <0.001 |
VP16–CREBhigh On/Off (n = 22, 5) | −60 ± 1.6 | 168 ± 12.5 | 23 ± 1.9 | 120 ± 1.3 | 0.99 ± 0.04 | 316 ± 9.9 | −39 ± 0.5 | 224 ± 15.9 | 36 ± 3.3 |
Wild-type On/Off (n = 19, 4) | −61 ± 1.7 | 157 ± 10.4 | 23 ± 1.0 | 120 ± 1.2 | 1.01 ± 0.03 | 306 ± 6.0 | −39 ± 0.8 | 231 ± 15.6 | 44 ± 3.5 |
p | 0.77 | 0.51 | 0.91 | 0.93 | 0.74 | 0.40 | 0.84 | 0.27 | 0.11 |
VP16–CREBhigh Off (n = 13, 2) | −61 ± 2.1 | 137 ± 10.0 | 18 ± 1.5 | 116 ± 2.2 | 0.89 ± 0.04 | 310 ± 14.7 | −40 ± 1.1 | 211 ± 16.6 | 45 ± 8.6 |
Wild-type Off (n = 19, 3) | −57 ± 1.4 | 134 ± 10.3 | 19 ± 1.2 | 119 ± 1.2 | 0.86 ± 0.03 | 321 ± 8.4 | −39 ± 1.0 | 243 ± 13.2 | 47 ± 7.6 |
p | 0.13 | 0.95 | 0.61 | 0.14 | 0.43 | 0.40 | 0.45 | 0.12 | 0.88 |
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τ, Membrane time constant; AP50, AP duration at 50% amplitude; dV/dtmax, maximum rate of depolarization. In VP16–CREBhigh On mice, the recordings were performed 10 d after transgene expression. In VP16–CREBhigh On/Off mice, the transgene was expressed for 10 d and then silenced for 10 additional days. In both cases, transgene expression was turned on when the mice were 4 weeks old. VP16–CREBhigh Off mice were 10 weeks old when the experiments were performed. In all these experiments, wild-type mice were housed and maintained in the same conditions than their mutant littermates.